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Chitinase and B-1,3-glucanase in the lutoid-body fraction of Hevea latex (Record no. 55562)

MARC details
000 -LEADER
fixed length control field	01960nam a2200217Ia 4500
100 ## - MAIN ENTRY--AUTHOR NAME
Personal name	Subroto T
245 #0 - TITLE STATEMENT
Title	Chitinase and B-1,3-glucanase in the lutoid-body fraction of Hevea latex
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Name of publisher	Phytochemistry
Year of publication	1996
300 ## - PHYSICAL DESCRIPTION
Number of Pages	29-37
500 ## - GENERAL NOTE
General note	Source Year: 1997
520 ## - SUMMARY, ETC.
Summary, etc	The lutoid-body fraction of latex from Hevea brasiliensis contains a limited number of major proteins (hevein, its precursor and the C-terminal fragment of this precursor, proteins with enzymic activities : 3 hevamine components, which are basic, vacuolar, chitinases with lysozyme activity, and a B-1,3-glucanase). Lutoid-body fractions from 3 clones (GT 1, PR 261 and LCB 1320) differed in their contents of these proteins. The hevamine components and glucanase were isolated and several enzymic and structural properties were investigated. These enzymes are basic proteins that cause coagulation of the negatively charged rubber particles. The coagulation occures in a rather narrow range of ratios of added protein to rubber particles, which indicates that charge neutralization is the determining factor. Differences in coagulation of rubber particles by lutoid-body fractions from different clones can be explained by their content of hevamine and glucanase. Glucanase from the lutoid-body fraction may dissolve callus tissue and this may explain the observation that clones with a high glucanase content produce more latex than clones with little glucanase. Sequence studies of 2CNBr peptides of the glucanase indicate that this protein is homologous with glucanase from other plants, and that a C-terminal peptide, possibly involved in vacuolar targeting, may have been cleaved off.
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical Term	Hevea brasiliensis
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical Term	Hevea latex
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name	Beintema J J
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name	Koningsveld G A van
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name	Schreuder H A
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name	Soedjanaatmadja U M S
856 ## - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier	IRRDB
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type	Journals

Holdings
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			Journals	RRII Library	RRII Library	Physiology	17/12/2021	Volume 43, Issue 1	Journals