Characterization of tonoplast pyrophosphatase from Hevea brasiliensis latex (Record no. 55454)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 01666nam a2200229Ia 4500 |
| 100 ## - MAIN ENTRY--AUTHOR NAME | |
| Personal name | Siswanto |
| 245 #0 - TITLE STATEMENT | |
| Title | Characterization of tonoplast pyrophosphatase from Hevea brasiliensis latex |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Name of publisher | Indian Journal of Natural Rubber Research |
| Year of publication | 1994 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Number of Pages | 8-Jan |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc | A pyrophosphatase (PPase) is evidenced on the lutoidic membrane in Hevea brasiliensis latex. Presence of Mg2+ is needed during the membrane washing, to preserve the tonoplastic enzyme activity. PPase functioning is Mg2+ and pyrophosphate (PPi)Km are measured. The substrate affinity is high (50uM). It is relatively thermosensitive. Many products were tested on the PPase functioning. K+ is a strong activator. It increases the reaction activity but does not modify the enzyme affinity. Among the monovalent cations, only Rb+ has a similar efffect. No divalent cation can take the place of Mg2+, but some of them such as Ca2+ or Mn2+ are inhibitors. Inhibition induced by cysteine, pCMB and NEM indicates the importance of SH groups in the enzyme active site. DIDS which breaks anion transport, inhibits the enzyme. DCCD and NaNO3, inhibitors of lutoidic ATPase, have no effect on the lutoidic PPase. Taking the results into account, it is possible to compare latex tonoplastic and cystolic PPases. The physiological role of this tonoplastic PPase is discussed. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Hevea brasiliensis |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Latex |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Lutoid |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Pyrophosphatase |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Rubber synthesis |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Tonoplast |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Clement A |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Jacob J L |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Prevot J C |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Journals |
| Withdrawn status | Lost status | Damaged status | Not for loan | Home library | Current library | Date acquired | Serial Enumeration / chronology | Koha item type |
|---|---|---|---|---|---|---|---|---|
| Journals | RRII Library | RRII Library | 17/12/2021 | Volume 7, Issue 1 | Journals |