Characterization of a full-length cDNA clone encoding glutamine synthetase from rubber tree latex (Record no. 55409)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 01621nam a2200217Ia 4500 |
| 100 ## - MAIN ENTRY--AUTHOR NAME | |
| Personal name | Pujade-Renaud V |
| 245 #0 - TITLE STATEMENT | |
| Title | Characterization of a full-length cDNA clone encoding glutamine synthetase from rubber tree latex |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Name of publisher | Plant Physiology and Biochemstry |
| Year of publication | 1997 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Number of Pages | 85-93 |
| 500 ## - GENERAL NOTE | |
| General note | Source Year: 1997 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc | A full-length cDNA clone (HbGS) encoding glutamine synthetase [glutamate-ammonia ligase] (GS, EC 6.3.1.2) was isolated from a lambda Zap II library of ethylene-treated Hevea brasiliensis latex. Sequence analysis of this clone revealed an open reading frame corresponding to a 356 amino acid polypeptide. The deduced amino acid sequence showeed 80-96;and 76;identity respectively with cytosolic and chloroplastic GS published sequences. These homologies and the lack of a N-terminal leader peptide sequence strongly suggested that this clone encodes a cytosolic glutamine synthetase. Southern blot analysis demonstrated the existence of an intron(s) and suggested that the rubber tree GS is encoded by a small multigene family. The short-term kinetics of ethylene action on gene expression revealed marked accumulation of the corresponding GS mRNA in the latex cells of ethylene-treated newly tapped trees, compared with non-treated controls. Additionally, tapping itself was shown to slightly stimulate GS gene expression. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Full-length cDNA |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Rubber latex |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Chrestin H |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Guern J |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Lacrotte R |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Perrot-Rechenmann C |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | PBA |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Journals |
| Withdrawn status | Lost status | Damaged status | Not for loan | Home library | Current library | Shelving location | Date acquired | Serial Enumeration / chronology | Koha item type |
|---|---|---|---|---|---|---|---|---|---|
| Journals | RRII Library | RRII Library | Physiology | 17/12/2021 | Volume 35, Issue 2 | Journals |