Characterisation of type 2 and 3 metallothioneins from Hevea brasiliensis bark (Record no. 55385)

MARC details
000 -LEADER
fixed length control field 01382nam a2200193Ia 4500
100 ## - MAIN ENTRY--AUTHOR NAME
Personal name Bahari Azlina et al.
245 #0 - TITLE STATEMENT
Title Characterisation of type 2 and 3 metallothioneins from Hevea brasiliensis bark
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Name of publisher Jouranl of Rubber Research
Year of publication 2013
300 ## - PHYSICAL DESCRIPTION
Number of Pages 257-270
520 ## - SUMMARY, ETC.
Summary, etc A small collection of ESts was generated from the bark of the rubber tree clone RRIM 2025. Low molecular weight metal-binding proteins, metallothioneins(MTs), were found to be very abundant in the bark gene expression profile. Multiple sequence alignment and phylogenetic analysis between three Hevea MTs and other plant MTs identified then as type 2 and type 3 proteins. Semi-quantitative PCR analysis indicated that each MT was differentially expressed between bark, leaves and latex, with the lowest transcript abundance in latex. In latex from ethephon-treated trees, all MT isoforms were induced at four hours after treatement while only one of them showed decreased expression sfter 24 hours. Our findings indicate the existence of a multiple gene family for Hevea MTs and Varying roles played by isoforms derived from different rubber tree tissues.
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical Term Bark
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Topical Term DNA sequencing
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Topical Term EST
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Topical Term Hevea brasiliensis
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Topical Term IsoformGene expression
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Topical Term Metallothionein
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Journals
Holdings
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