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Association of a viroid with tapping panel dryness of Hevea brasiliensis and its molecular characterization (Record no. 54568)

MARC details
000 -LEADER
fixed length control field	02052nam a2200217Ia 4500
100 ## - MAIN ENTRY--AUTHOR NAME
Personal name	Abraham T
245 #0 - TITLE STATEMENT
Title	Association of a viroid with tapping panel dryness of Hevea brasiliensis and its molecular characterization
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Name of publisher	Sustainability of the NR Industry: Issues, Challenges & Opportunities: International Rubber Conference, 21-22 November 2016, Cambodian Rubber Research Institute (CRRI) and International Rubber Research and Development Board (IRRDB), Siem Reap, Cambodia, pp.396-408.
520 ## - SUMMARY, ETC.
Summary, etc	Tapping panel dryness (TPD) syndrome is a serious malady of rubber (Hevea brasiliensis) with unknown etiology occurring in all rubber growing areas resulting in partial to complete drying of tapping panel. The possibility of association of viroid with TPD syndrome was investigated in the present study. An infectious LMW RNA was detected from different samples drawn from varying ages of trees from different locations. The consistency in the presence of LMW RNA over three years of observation in TPD affected trees and its absence in majority of the healthy ones points to the association of the LMW RNA with TPD. About 30 per cent of the apparently healthy trees which may be symptomless carriers also showed presence of LMW RNA. Such trees later showed TPD symptoms. Presence of bands in apparently healthy samples indicates that the biotic agent can be detected in the tree much before the TPD symptoms are visible. The PCR of the cDNA using viroid specific primers consistently amplified products in the range of viroids in R-PAGE positive TPD affected samples but was absent in R-PAGE negative apparently healthy samples. The amplified LMW RNA showed 98;sequence homology to Potato Spindle Turber Viroid (PSTVd) on BLAST analysis.
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical Term	LMW RNA
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical Term	Symptomless carriers
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical Term	TPD
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical Term	Viroid
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name	Jacob C K
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name	Kumar A
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name	Malathi V G
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name	Mathew J
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name	Ramachandran P
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type	Journals

Holdings
Withdrawn status	Lost status	Damaged status	Not for loan	Home library	Current library	Shelving location	Date acquired	Koha item type
			Journals	RRII Library	RRII Library	Pathology	01/01/2021	Journals