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Antifungal action of Hevea brasiliensis latex. Its effect in combination with fluconazole on Candida albicans growth (Record no. 54319)

MARC details
000 -LEADER
fixed length control field	01944nam a2200217Ia 4500
100 ## - MAIN ENTRY--AUTHOR NAME
Personal name	Giordani R
245 #0 - TITLE STATEMENT
Title	Antifungal action of Hevea brasiliensis latex. Its effect in combination with fluconazole on Candida albicans growth
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Name of publisher	Mycoses
Year of publication	1999
300 ## - PHYSICAL DESCRIPTION
Number of Pages	465-474
500 ## - GENERAL NOTE
General note	Source Year: 2000
520 ## - SUMMARY, ETC.
Summary, etc	Latex from H. brasiliensis and its subcellular fractions (L-serum and C-serum) were tested for antifungal activity alone or in combination with fluconazole. C.albicans growth was inhibited with the same efficacy when yeasts were inoculated into culture medium supplemented over the total growth phase with latex as when latex was added during the exponential phase only; the minimum inhibitory concentration (MIC 80;) of H. brasiliensis latex was 123 micro g/ml. By means of a non-linear regression analysis of the experimental data, 2 distinct fixation sites for fluconazole (FCZ) were determined: one of strong affinity (Kaff=0.0162 micro g/ml) and another of low aminity (Kaff=0.0071 micro g/ml). After addition of a mixture of FCZ and latex during the exponential phase, the affinity constant of yeasts for FCZ was calculated; when latex was in a final concentration of 21 micro g/ml (Kaff= 1 micro g/ml) or 42 micro g/ml (Kaff=0.277 micro g/ml) and without latex (Kaff=0.0502 micro g/ml). In 2 cases a synergistic effect between latex and FCZ was obtained. The highest efficacy was obtained with a latex concentration of 21 micro g/ml. The addition of subcellular fractions of latex, L-serum and C-serum, did not cause an antifungal effect. Electron microscopy observations indicated a limited cell wall degradation and a high percentage of coagulated yeasts.
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical Term	Antifungal action
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical Term	Hevea brasiliensis
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name	Buc J
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name	Gachon C
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name	Jacob J L
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name	Regli P
856 ## - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier	IRRDB
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type	Journals

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