An Approach to identify disease resistance gene analogues in Hevea (Record no. 54100)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 01407nam a2200241Ia 4500 |
| 100 ## - MAIN ENTRY--AUTHOR NAME | |
| Personal name | Licy J |
| 245 #3 - TITLE STATEMENT | |
| Title | An Approach to identify disease resistance gene analogues in Hevea |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Name of publisher | Indian Journal of Natural Rubber Research |
| Year of publication | 2000 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Number of Pages | 79-85 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc | A preliminary attempt was made to identify possible disease resistance gene analogues in rubber. A procedure was developed for the extraction of high quality DNA from rubber leaves, which can be used for the polymerase chain reaction (PCR) amplification of DNA. Eighteen primers, designed based on homologies between known resistance genes, were used in various combinations to amplify sequences from rubber cultivar FX 516, which is resistant to Phytophthora leaf fall disease and cultivar RRII 105, which is tolerant. The PCR products were cloned into plasmid vectors and the cloned inserts were sequenced. Although none of the clones obtained had high homology to resistance gene sequences, the putative protein encoded by one sequence had some homology to hem N gene. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Cloning |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Hevea |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Phytophtora leaf fall |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | POlymerase chain reaction |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Resistance genes |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Bligh F J |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Davey M R |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Dickinson J |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Power J B |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Saraswathyamma C K |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Journals |
| Withdrawn status | Lost status | Damaged status | Not for loan | Home library | Current library | Shelving location | Date acquired | Serial Enumeration / chronology | Koha item type |
|---|---|---|---|---|---|---|---|---|---|
| Journals | RRII Library | RRII Library | Botany | 17/12/2021 | Volume 13, Issue 02-Jan | Journals |