Agrobacterium-mediated transformation of Hevea anther calli and their regeneration into plantlets (Record no. 53976)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 01489nam a2200205Ia 4500 |
| 100 ## - MAIN ENTRY--AUTHOR NAME | |
| Personal name | Arokiaraj P |
| 245 #0 - TITLE STATEMENT | |
| Title | Agrobacterium-mediated transformation of Hevea anther calli and their regeneration into plantlets |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Name of publisher | Journal of Natural Rubber Research |
| Year of publication | 1996 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Number of Pages | 77-87 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc | A gene transfer system for Hevea brasiliensis was estabilished with Agrobacterium tumefaciens GV 2260 (p35SGUSINT) and LBA4404 (pAL4404/pMON9793). In this system Hevea brasiliensis anther-derived calli were transformed with vectors harbouring the B-glucuronidase (gus) gene and the neomycin phosphotransferase (nptII) gene. The success of gene transfer was determined by histochemical staining and fluorometric assay for B-glucuronidase activity and enzyme linked immunosorbent assay for detecting neomycin phosphotransferase II protein levels. These indepedent assays all showed a several-fold increase, compared to control values, in enzyme activity and protein levels in extracts from transformed calli and embryoids of Hevea brasiliensis. The presence of the gus gene in transformed plantlets was confirmed by using the polymerase chain reaction protocol as performed on DNA isolated from transformed calli and embryoids. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Agrobacterium |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Anther calli |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Hevea brasiliensis |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Charlwood B V |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Coomber S |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Jaafar H |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Jones H |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Journals |
| Withdrawn status | Lost status | Damaged status | Not for loan | Home library | Current library | Shelving location | Date acquired | Serial Enumeration / chronology | Koha item type |
|---|---|---|---|---|---|---|---|---|---|
| Journals | RRII Library | RRII Library | Biotechnology | 17/12/2021 | Volume 11, Issue 2 | Journals |