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Activity of nitrogen assimilating enzymes in young rubber trees grown with different ratios of nitrate and ammonium (Record no. 53772)

MARC details
000 -LEADER
fixed length control field	02004nam a2200193Ia 4500
100 ## - MAIN ENTRY--AUTHOR NAME
Personal name	Lemos G B De
245 #0 - TITLE STATEMENT
Title	Activity of nitrogen assimilating enzymes in young rubber trees grown with different ratios of nitrate and ammonium
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Name of publisher	Revista Brasileria de Fisiologia Vegetal
Year of publication	1999
300 ## - PHYSICAL DESCRIPTION
Number of Pages	113-118
500 ## - GENERAL NOTE
General note	Source Year: 2000
520 ## - SUMMARY, ETC.
Summary, etc	The effects of different NO3-/NH4+ proportions (0/0, 8/0, 6/2, 4/4, 2/6 or 0/8 mM) on the concentration of total N and soluble protein, and on the activities of nitrate reductase (RN, E.C.1.6.6.1), glutamine synthetase (GS, E.C. 6.3.1.2) and glutamate synthase (Fd-GOGAT, E.C. 1.4.1.7 and NADH-GOGAT, E.C. 1.4.1.14) in roots and leaves of young Hevea brasiliensis trees were investigated. The experiment was carried out under controlled environmental conditions for 120 days, in plastic pots containing 4 litres of washed sand. Bolle-Jones nutrient solution, at pH 6, was prepared to give the desired NO3-/NH4+ ratio, significantly increased total N concentration in leaves, stems and roots. The activities of GOGAT in leaves and roots and of GS in leaves did not differ between plants treated exclusively with either NO3-/NH4+ , but were significantly higher than in plants not treated with N. In roots, the activities of NR and GS were higher in plants treated exclusively with NO3- and decreased significantly when NH4+ was added to the nutrient solution. No NR activity was detected in leaves in this experiment, suggesting that in young rubber trees, NO3- reduction and primary NH4+ assimilation take place only in roots. Because NO3- reduction was absent in the leaves, it is hypothesized that the main role of the high GS and GOGAT activities found in the leaves is to reassimilate NH4+ released during photorespiration.
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical Term	Nitrogen assimilation
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name	Delu Filho N
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name	Oliveira L E M De
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name	Purcino A A C
856 ## - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier	CPA
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type	Journals

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			Journals	RRII Library	RRII Library	Soils and agonomy	17/12/2021	Volume 11, Issue 2	Journals