A Novel cDNA from Parthenium argentatum gray enhances the rubber biosynthetic activity in vitro (Record no. 53482)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02219nam a2200229Ia 4500 |
| 100 ## - MAIN ENTRY--AUTHOR NAME | |
| Personal name | Kim In Jeong |
| 245 #2 - TITLE STATEMENT | |
| Title | A Novel cDNA from Parthenium argentatum gray enhances the rubber biosynthetic activity in vitro |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Name of publisher | Journal of Experimental Botany |
| Year of publication | 2004 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Number of Pages | 377-385 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc | Natural rubber (cis-1,4-polyisoprene) is an isoprenoid compound produced exclusively in plants by the action of rubber transferase. Despite a keen interest in revealing the mechanisms of rubber chain elongation and chain length determination, the molecular nature of rubber transferase has not yet been identified. A recent report has revealed that a 24 kDa protein tightly associated with the small rubber particles of Hevea brasiliensis, therefore designated small rubber particle protein (SRPP), plays a positive role in rubber biosynthesis. Since guayule (Parthenium argentatum gray) produces natural rubber similar in size to H. brasiliensis, it is of critical interest to investigate whether guayule contains a similar protein to the SRPP. A cDNA clone has been isolated in guayule that shares a sequence homology with the SRPP, thus designated guayule homologue of SRPP (GHS), and the catalytic function of the protein was characterized. Sequence analysis revealed that the GHS is highly homologous in several conserved regions to the SRPP (50;identity). In vitro functional analysis of the recombinant protein overexpressed in E.coli revealed that the GHS plays a positive role in isopentenyl diphosphate incorporation into high molecular weight rubbers as SRPP does. These results indicate that guayule and Hevea rubber trees contain a protein that is similar in its amino acid sequence and plays a role in isopentenyl diphosphate incorporation in vitro, implying that it contributes to the enhancement of rubber biosynthetic activity in rubber trees. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Guayule |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Hevea brasiliensis |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | IDP incorporation |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Parthenium argentatum |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Rubber biosynthesis |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Rubber particle protein |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Kang Hunseung |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Kwak Yeon Sig |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ryu Stephen B |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Journals |
| Withdrawn status | Lost status | Damaged status | Not for loan | Home library | Current library | Shelving location | Date acquired | Serial Enumeration / chronology | Koha item type |
|---|---|---|---|---|---|---|---|---|---|
| Journals | RRII Library | RRII Library | Biotechnology | 17/12/2021 | Volume 55, Issue 396 | Journals |