3-Hydroxy-3-methylglutaryl-coenzyme, a synthase in Hevea brasiliensis (Record no. 53247)

MARC details
000 -LEADER
fixed length control field 01612nam a2200181Ia 4500
100 ## - MAIN ENTRY--AUTHOR NAME
Personal name Suvachittanont W
245 #0 - TITLE STATEMENT
Title 3-Hydroxy-3-methylglutaryl-coenzyme, a synthase in Hevea brasiliensis
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Name of publisher Phytochemistry
Year of publication 1995
300 ## - PHYSICAL DESCRIPTION
Number of Pages 757-761
500 ## - GENERAL NOTE
General note Source Year: 1996
520 ## - SUMMARY, ETC.
Summary, etc 3-Hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) synthase activity in rubber (H. brasiliensis) latex (collected from 15-year-old trees of clone RRIM 600 growing at the Songkal Rubber Research Centre) and in fractions of latex obtained by centrifugation was deterrmined using a radiochemical method. The enzyme was found in both C-serum and bottom fraction, but most was in the C-serum. H. brasiliensis leaves showed very low enzyme activity. HMG-CoA synthase in C-serum was free of HMG-CoA lyase. The enzyme in C-serum was stable at -70 C. The formation of HMG-CoA did not depend on a supply of exogenous acetoacetyle-CoA, and the rate of formation was increased as the concentration of acetyl-CoA was increased. The Km for acetyl-CoA was 9mM. The enzyme in C-serum was inhibited by several divalent cations, p-chloromercuribenzoate and dithiothreitol. Diurnal variation in the activity of the enzyme in both C-serum and extracts of leaves was observed. A positive correlation between the enzyme activity and the rubber content of the latex suggested that the enzyme regulates the synthesis of rubber in the latex.
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical Term 3-hydroxy-3-methylglutaryl-coenzyme
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical Term Hevea brasiliensis
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Wititsuwannakul R
856 ## - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier HOA
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Journals
Holdings
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