Identification of DNA polymorphism among clones of Hevea brasiliensis muell. arg. using RAPD analysis (Record no. 47072)

MARC details
000 -LEADER
fixed length control field 01967nam a2200289Ia 4500
100 ## - MAIN ENTRY--AUTHOR NAME
Personal name Venkatachalam P
245 #0 - TITLE STATEMENT
Title Identification of DNA polymorphism among clones of Hevea brasiliensis muell. arg. using RAPD analysis
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Name of publisher Indian Journal of Natural Rubber Research
Year of publication 2002
300 ## - PHYSICAL DESCRIPTION
Number of Pages 172-181
520 ## - SUMMARY, ETC.
Summary, etc RAPD (Random amplified polymorphic DNAs) were used to study the DNA polymorphism in 37 Hevea clones representing variability for several morphological, physiological and other characters. Eighty random sequenced 10-mer primers were useed of which nine produced clear and scorable bands while eight produced polymorphic amplification products between 300 to 4000 base pairs in size, sufficient to distinguish between the clones. Of bands from the 8 primers 51.5;were polymorphic in the Hevea clones studied. A dendrogram developed using Jaccard's coefficients indicated genetic relationships among these clones. Most of the primary clones were clustered together in the dendrogram. UPGMA cluster analysis indicated that some of the clones are genetically close although they have been developed from different breeding programmes. The presence of polymorphic bands in the genomic DNA was further confirmed by Southern blot analysis. Detection of DNA polymorphism in the Hevea clones opens up the possibility of development of molecular map. This molecular approach will be useful for developing marker-assisted selection tools for genetic improvement of Hevea.
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical Term DNA polymorphism
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Topical Term DNA polymorphism
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Topical Term Hevea brasiliensis
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Topical Term Hevea brasiliensis
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Topical Term Random oligonucleotide
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Topical Term Random oligonucleotide
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Topical Term RAPD
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Topical Term RAPD
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Gireesh T
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Personal name Priya P
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Saraswathyamma C K
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Thanseem I
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Thomas S
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Thulaseedharan A
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Articles
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