cDNA cloning and expression analysis of three components of SCFco11 complex in Hevea brasiliensis
Ma Rui-Feng
cDNA cloning and expression analysis of three components of SCFco11 complex in Hevea brasiliensis - Journal of Rubber Research 2012 - 141-152
Source Year: 2012
To gain a deeper understanding of jasmonate signalling in specific tissues, three of full-length cDNAs, refferred to as HbCUL1, HbSKP1 and HbRBX1 were isolated from laticifers of rubber tree by the rapid amplification of cDNA ends (RACE) method and analysed by sequence analysis and real-time RT-PCR. The putative proteins, HbCUL1, HbSKP1 and HbRBX1, specific domains of CULLIN superfamily and Cullin Nedd8, Skp1-POZ and Skp1, and RING superfamily, respectively. HbCUL1 and HbSKP1 were most related to AtCUL1 and AtASK1 among the members of Cullins and ASKs from Arabidopsis, respectively. Methyl jasmonate was more effective than ethylene on up-regulating the expression of HbCUL1, HbSKP1 and HbRBX1. These data suggest that HbCUL1, HbSKP1 and HbRBX1 may be the invariant components of SCFc011 complex of jasmonate signaling in laticifers of rubber tree.
Hevea brasiliensis Muell. Arg
Jasmonate signalling
Laticifer
cDNA cloning and expression analysis of three components of SCFco11 complex in Hevea brasiliensis - Journal of Rubber Research 2012 - 141-152
Source Year: 2012
To gain a deeper understanding of jasmonate signalling in specific tissues, three of full-length cDNAs, refferred to as HbCUL1, HbSKP1 and HbRBX1 were isolated from laticifers of rubber tree by the rapid amplification of cDNA ends (RACE) method and analysed by sequence analysis and real-time RT-PCR. The putative proteins, HbCUL1, HbSKP1 and HbRBX1, specific domains of CULLIN superfamily and Cullin Nedd8, Skp1-POZ and Skp1, and RING superfamily, respectively. HbCUL1 and HbSKP1 were most related to AtCUL1 and AtASK1 among the members of Cullins and ASKs from Arabidopsis, respectively. Methyl jasmonate was more effective than ethylene on up-regulating the expression of HbCUL1, HbSKP1 and HbRBX1. These data suggest that HbCUL1, HbSKP1 and HbRBX1 may be the invariant components of SCFc011 complex of jasmonate signaling in laticifers of rubber tree.
Hevea brasiliensis Muell. Arg
Jasmonate signalling
Laticifer