Problems associated with the estimation of cyanide in rubber seed kernel
Mallika G V
Problems associated with the estimation of cyanide in rubber seed kernel
The cyanide content of rubber seeds was estimatd by autolysis techniques. Autolysis of random seed samples yielded total cyanide contents ranging from 100 to 4000 mg/kg. Six samples collected from the same field, one day after seed fall (in an attempt to limit the variation), yielded an average cyanide content of 1819 mg/kg. Comparison of seeds from the same fruit found wide variations in cyanide content, e.g. 15-1897 mg/kg in one fruit, indicating a need for large seed samples. An optimum sample size of 6-8 g is suggested. Hydrolysis with trichloroacetic acid [TCA] for 4 h yielded <3;of the total cyanide. Using 2M H2SO4 at room temperature also yielded very little. Acid reflux for 30 h yielded the full amount, but autolysis took only 2 h to do the same. Adding exogenous linamarase to distilled autolysate residue did not release any further cyanide.
Cyanogenic glucoside estimation
Rubber seed
Rubber seed kernel
Problems associated with the estimation of cyanide in rubber seed kernel
The cyanide content of rubber seeds was estimatd by autolysis techniques. Autolysis of random seed samples yielded total cyanide contents ranging from 100 to 4000 mg/kg. Six samples collected from the same field, one day after seed fall (in an attempt to limit the variation), yielded an average cyanide content of 1819 mg/kg. Comparison of seeds from the same fruit found wide variations in cyanide content, e.g. 15-1897 mg/kg in one fruit, indicating a need for large seed samples. An optimum sample size of 6-8 g is suggested. Hydrolysis with trichloroacetic acid [TCA] for 4 h yielded <3;of the total cyanide. Using 2M H2SO4 at room temperature also yielded very little. Acid reflux for 30 h yielded the full amount, but autolysis took only 2 h to do the same. Adding exogenous linamarase to distilled autolysate residue did not release any further cyanide.
Cyanogenic glucoside estimation
Rubber seed
Rubber seed kernel