Hevea brasiliensis is the primary source of natural rubber and its seeds are being used as an important source of stock seedlings for propagation and potential raw material for non-edible oil production. The present study deals with assessment of seed trait variability in seeds of RRII 400 series clones of H. brasiliensis viz., RRII 414, RRII 417, RRII 422, RRII 429 and RRII 430. The results demonstrated that seeds of RRII 414 had the highest dry weight (4.5 g), followed by RRII 417 (3.3 g), RRII 429 (3.3 g), RRII 430 (3.3 g) and RRII 422 (3.0 g). The highest kernel dry weight was recorded by the seeds of clone RRII 414 (2.4 g). Positive correlation was found between total seed weight and kernel weight of seeds. RRII 429 registered the highest percentage of germination (72 %) followed by RRII 422 (59 %) and RRII 414 (58 %). The study showed that the seed weight could be used as an indicator of kernel biomass in sorting seeds for commercial oil production and stock seedling generation.