Identification of microsatellites from Hevea brasiliensis was initiated by the construction of genomic DNA insert library in lambda ZAP Express vector. 10,000 plaques were screened in total and 113 (CT)n and 91 (AC)n positive clones/plaques were selected from the primary plates. Out of the 204 positive clones, 50 clones were selected at random and subjected to a second round of screening. After secondary screening, 30 clones were recovered individually of which 16 were (CT/GA)n and 14 (AC/TG)n. In vivo excesion of the phagemid containing the genomic DNA insert was done from these 30 positive clones. The isolated phagemids were restricted with Xba I and Bam H I to release the inserts as well as to identify the duplicate clones through restriction profiles. Screening was also performed with the dinucleotide repeat probe (AT/TA)20 and no positive signal was obtained possibly due to the minimum occurrence of this repeat sequence within the genomic DNA. 12 positive clones were sequenced to validate the presence of microsatellites from genomic libraries of Hevea. In total, none of the clones were identified with microsatellite repreats. Eight of the positive clones contained long arrays of the dinucleotide motifs CG, TG, AG, CA, CT, where as one positive clone possessed trinucleotide repeats GAT, GTT and one had a tetranucleotide repeat AAAT. Both simpple and compound repeats were observed. Identification and characterization of these SSR containing genomic clones is the initial step towards the development of microsatellite markers in Hevea.