The effect of thidiazuron on axillary shoot proliferation of Hevea brasiliensis in vitro
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TextPublication details: Journal of the Rubber Research Institute of Sri Lanka 1996Description: 14-JanSubject(s): Summary: Multiple axillary shoot production with a shoot doubling time of 26 days was obtained from nodal explants of juvenile origin Hevea brasiliensis on a medium containing thidiazuron (N-phenyl- N 1, 2, 3- thidiazol-5- ylyrea) at 0.02 ppm in combination with NAA at 0.2 ppm. The clusters of axillary buds produced in the presence of thidiazuron were subcultured at 4-week intervals while subdividing into two to four small propagules depending on the size of the cluster. Axillary buds showed a satisfactory elongation on their transfer onto a growth regulator-free medium. Elongated axillary shoots which were more than 10 mm in height produced roots in the presence of IBA at 2 ppm.
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RRII Library Biotechnology | Volume 77, Issue | Journals |
Multiple axillary shoot production with a shoot doubling time of 26 days was obtained from nodal explants of juvenile origin Hevea brasiliensis on a medium containing thidiazuron (N-phenyl- N 1, 2, 3- thidiazol-5- ylyrea) at 0.02 ppm in combination with NAA at 0.2 ppm. The clusters of axillary buds produced in the presence of thidiazuron were subcultured at 4-week intervals while subdividing into two to four small propagules depending on the size of the cluster. Axillary buds showed a satisfactory elongation on their transfer onto a growth regulator-free medium. Elongated axillary shoots which were more than 10 mm in height produced roots in the presence of IBA at 2 ppm.
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